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免疫共沉淀（Co-Immunoprecipitation）是以抗體和抗原之間的專一性作用為基礎(chǔ)的用于研究蛋白質(zhì)相互作用的經(jīng)典方法。是確定兩種蛋白質(zhì)在完整細(xì)胞內(nèi)生理性相互作用的有效方法。

基本原理：在細(xì)胞裂解液中加入抗原特異性的抗體,抗體、抗原以及與抗原具有相互作用的蛋白質(zhì)通過抗原與抗體之間的免疫反應(yīng)形成免疫復(fù)合物,洗脫收集復(fù)合物,SDS-PAGE電泳,western blot或質(zhì)譜可驗證或鑒定與抗原互作的蛋白質(zhì)。

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