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甲苯胺藍染色

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軟骨基質(zhì)對甲苯胺藍的異染性，是因為軟骨基質(zhì)的主要成分是軟骨黏蛋白、多糖物質(zhì)，而酸性硫酸根與嗜堿性染料有親和力。此法用于顯示新生骨組織鈣鹽沉積，成骨細胞數(shù)目統(tǒng)計，觀察軟骨退變，軟骨細胞壞死鈣化，軟骨基質(zhì)裂隙，膠原纖維增生等病理改變。染色結(jié)果：軟骨和成骨細胞呈藍紫色，背景呈淡藍色。

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